Additional Cell Culture Reagents and Supplements
Amsbio LLC (formerly Genlantis) Subculturing Reagents Kit
Functionally tested subculturing reagent for primary cells
STEMCELL Technologies ImmunoCult™ Human CD3/CD28 T Cell Activator
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens.This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. 1. Antibody complexes bind to and cross-link CD3 and CD28 cell surface ligands, thereby providing the required signals for T cell activation. ImmunoCult™ Human CD3/CD28 T Cell Activator can be used on the Seahorse XF Analyzer to measure T cell activation response and is also available as part of the Agilent Seahorse XF Hu T Cell Activation Assay Kit.This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an AM under an approved investigational new drug or clinical trial application.
STEMCELL Technologies Anti-Adherence Rinsing Solution, Size: 100 mL
Formerly known as AggreWell™ Rinsing Solution, name change effective Dec 2017.Anti-Adherence Rinsing Solution is a surfactant solution for pre-treating cultureware to reduce surface tension and prevent cell adhesion. It can be used with plates, flasks, pipettes, strainers, and other cultureware. Anti-Adherence Rinsing Solution is required for use with the following:• AggreWell™ plates (e.g. Catalog #34811) • Organoid growth media, including HepatiCult™ (Catalog #06030) and PancreaCult™ (Catalog #06040)
STEMCELL Technologies Heparin Solution, Size: 2 mL
Heparin is a mucopolysaccharide with anticoagulant properties. It supports the binding of fibroblast growth factor (FGF) to its receptor and increases the stability of FGF. Heparin is used together with epidermal growth factor (EGF) and FGF in NeuroCult™ media for the culture of embryonic rat or adult human neural stem and progenitor cells, and adult mouse neural stem and progenitor cells. For complete instructions, refer to the Technical Manual: In Vitro Proliferation and Differentiation of Neural Stem and Progenitor Cells Using NeuroCult™ (Human; Document #28724 or Mouse/Rat; Document #28725), available at www.stemcell.com or contact us to request a copy. Heparin Solution is also required as a supplement in various other STEMCELL culture media, including MammoCult™ Human Medium Kit (Catalog #05620), EpiCult™-B Mouse Medium Kit (Catalog #05610), and EC-Cult™-XF Culture Kit (Catalog #08000).
STEMCELL Technologies Hydrocortisone Stock Solution, Size: 3 mL
Hydrocortisone Stock Solution is recommended for use as a cell culture supplement and for the preparation of PneumaCult™, MammoCult™, and EpiCult™ media when culturing airway epithelial cells, mammary primary cells, or tumor cell lines.Hydrocortisone Stock Solution is provided at a concentration of 96 µg/mL, which is ideal as a 200X stock solution for use with PneumaCult™-ALI (Catalog #05001), MammoCult™ (Catalog #05620), EpiCult™-B (Human; Catalog #05601), and EpiCult™-C (Catalog #05630) media. It may also be used for preparing PneumaCult™-Ex Medium (Catalog #05008) or PneumaCult™-Ex Plus Medium (Catalog #05040).
FUJIFILM IRVINE SCIENTIFIC INC BalanCD HEK293 Feed, 500 mL
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.
STEMCELL Technologies 0.1% Gelatin in Water, Size: 500 mL
This product is recommended for the coating of culture dishes or flasks to grow mouse embryonic stem cells (mESCs), mouse induced pluripotent stem cells (miPSCs) or primary embryonic fibroblasts (PEFs).
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, Size: 2 mL
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble tetrameric antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material (AM) following the framework outlined in USP<1043>. Contact STEMCELL Technologies to learn how we can work with you to qualify this reagent as an AM under an approved investigational new drug (IND) or clinical trial application (CTA).
Sigma Aldrich Fine Chemicals Biosciences EmbryoMax Advanced KSOM Embryo Medium | 50mL
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
InVitria ITSE Animal-Free - 10mL
InVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular signalingTransferrin: delivers intracellular iron via the transferrin receptor, drives cellular respiration, chelates free iron, and reduces generation of cytotoxic free radicals and peroxideSelenium: required for the activity of antioxidant enzymes such as glutathione peroxidase and thioredoxin reductaseEthanolamine: phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of certain cell typesAlbumin: binds, transports, and delivers ions, fatty acids, vitamins, hormones, small-molecule drugs, and toxins; regulates serum pH and osmotic pressure; and provides antioxidant activityMade in the USA.
Key Scientific Products CryoCare, Organism Preservative System, Yellow Beads, 50 Vials per Box
CryoCare organism preservative system lowers the cost of stock cultures by stretching each expensive culture into 15 or more which meet NCCLS standards. Vial contains ceramic beads in broth base. Includes 50 vials per box with yellow beads.
General Laboratory Products Saline (0.9%) - 100ml, 12 vials per case
Saline (0.9%) - 100ml. Saline is routinely used a diluent. This is an industrial use product, not for humans and not to be used as an injectable. 12 Vials per case. Used as a diluent.